Identification and Functional Analysis of CAP Genes from the Wheat Stripe Rust Fungus Puccinia striiformis f. sp. tritici

Cysteine-rich secretory proteins (C), antigen 5 (A), and pathogenesis-related 1 (P) comprise widespread CAP superfamily proteins, which have been proven to be novel virulence factors of mammalian pathogenic fungi some plant pathogens. Despite this, the identification function in more species pathogens still need studied. This work presents functional analysis from Puccinia striiformis f. sp. tritici (Pst), an important fungal pathogen that causes wheat stripe rust on worldwide. A total six genes were identified Pst genome, designated as PsCAP1–PsCAP6. Five PsCAP including PsCAP1, PsCAP2, PsCAP3, PsCAP4, PsCAP5, N-terminal signal peptides secreted with yeast sequence trap assay. Single-nucleotide polymorphism (SNP) indicated they showed a low level intraspecies polymorphism. The expression abundance at different infection stages was detected by RT-qPCR, most them highly expressed during also sexual reproduction barberry (Berberis shensiana). Noticeably, silencing these BSMV-mediated HIGS PsCAP5 contribute significantly wheat. These results indicate may act infection, provides insights into pathogenicity.